Is there another step? Do I need to convert it to an optical transfer function? Am I missing something? I assume now that this 3D Array imported into Matlab is my PSF. (note: there may be an easier way to do this) So far, I have found that I can convert the TIFF to an HDF5 file (.h5) and import that with the following code: Once I have a 3D TIFF stack, I want to import this into MATLAB. Typically, I have been generating a TIFF stack of 0.1 micron bead, or a theoretical 3D PSFs using the excellent ImageJ plugin by Biomedical Imaging Group in Lausanne ( ).

I am familiar with constrained iterative deconvolution and have made 3D TIF stacks of beads before to generate PSFs in programs like Slidebook and Volocity etc. I need the PSFs for testing deblurring algorithms. I am new to coding and image analysis in Matlab and attempting to import theoretical or measured (0.1 micron bead) point-spread-functions (PSFs) in to Matlab.